(topic-hcs)=

# High Content Screening

High Content Screening (HCS) workflows record images for multiple conditions at the same time, often in multi-well plates. The OME-TIFF specification provided first-class support for HCS, and the OME-Zarr addressed HCS needs early on, with a [dedicated specification for plates and wells](https://ngff.openmicroscopy.org/0.5/#hcs-layout).

- The [Fractal analytics framework](https://fractal-analytics-platform.github.io/) for large scale processing with OME-Zarr has multiple [workflows to analyse HCS data](https://fractal-analytics-platform.github.io/fractal_tasks/) ([preprint](https://www.biorxiv.org/content/10.64898/2026.03.05.709921v1.full)).

- Massei, R., Busch, W., Serrano-Solano, B. et al. High-content screening (HCS) workflows for FAIR image data management with OMERO. Sci Rep 15, 16236 (2025). https://doi.org/10.1038/s41598-025-00720-0